Effect of photobiomodulation therapy on the morphology, intracellular calcium concentration, free radical generation, apoptosis and necrosis of human mesenchymal stem cells-an in vitro study.

The aim of the study was to investigate the impact of multiwave locked system (MLS M1) emitting synchronized laser radiation at 2 wavelength simultaneous (λ = 808 nm, λ = 905 nm) on the mesenchymal stem cells (MSCs). Human MSCs were exposed to MLS M1 system laser radiation with the power density 195-318 mW/cm2 and doses of energy 3-20 J, in continuous wave emission (CW) or pulsed emission (PE). After irradiation exposure in doses of energy 3 J, 10 J (CW, ƒ = 1000 Hz), and 20 J (ƒ = 2000 Hz), increased proliferation of MSCs was observed. Significant reduction of Fluo-4 Direct™ Ca2+ indicator fluorescence over controls after CW and PE with 3 J, 10 J, and 20 J was noticed. A decrease in fluorescence intensity after the application of radiation with a frequency of 2000 Hz in doses of 3 J, 10 J, and 20 J was observed. In contrary, an increase in DCF fluorescence intensity after irradiation with laser radiation of 3 J, 10 J, and 20 J (CW, ƒ = 1000 Hz and ƒ = 2000 Hz) was also shown. Laser irradiation at a dose of 20 J, emitted at 1000 Hz and 2000 Hz, and 3 J emitted at a frequency of 2000 Hz caused a statistically significant loss of MSC viability. The applied photobiomodulation therapy induced a strong pro-apoptotic effect dependent on the laser irradiation exposure time, while the application of a sufficiently high-energy dose and frequency with a sufficiently long exposure time significantly increased intracellular calcium ion concentration and free radical production by MSCs.

The effect of MLS laser radiation on cell lipid membrane.

INTRODUCTION: Authors of numerous publications have proved the therapeutic effect of laser irradiation on biological material, but the mechanisms at cellular and subcellular level are not yet well understood. OBJECTIVE: The aim of this study was to assess the effect of laser radiation emitted by the MLS M1 system (Multiwave Locked System) at two wavelengths (808 nm continuous and 905 nm pulsed) on the stability and fluidity of liposomes with a lipid composition similar to that of human erythrocyte membrane or made of phosphatidylocholine. MATERIAL AND METHODS: Liposomes were exposed to low-energy laser radiation at surface densities 195 mW/cm2 (frequency 1,000 Hz) and 230 mW/cm2 (frequency 2,000 Hz). Different doses of radiation energy in the range 0-15 J were applied. The surface energy density was within the range 0.46 - 4.9 J/cm 2. RESULTS: The fluidity and stability of liposomes subjected to such irradiation changed depending on the parameters of radiation used. CONCLUSIONS: Since MLS M1 laser radiation, depending on the parameters used, affects fluidity and stability of liposomes with the lipid content similar to erythrocyte membrane, it may also cause structural and functional changes in cell membranes.

[Comparison of the analgesic effect of interferential current (IFC) and TENS in patients with low back pain]. / Porównanie dzialania przeciwbólowego pradów interferencyjnych i TENS u pacjentów z dolegliwosciami bólowymi w czesci ledzwiowo-krzyzowej kregoslupa.

INTRODUCTION: Electrotherapy, including theTENS and interferential current (IFC) is one of the most frequently used treatments in physical therapy in patients with low back pain. The aim of this study was to assess the influence of TENS and IFC on pain relief and to compare the analgesic efficacy of the two currents. MATERIAL AND METHODS: Sixty patients aged 53.5 ± 12.5, with low back pain, were randomly divided into two groups: IFC (gr. I) and TENS (gr. II). Depending on the groups, patients were given series of ten 20-minute sessions using either IF orTENS currents. In all patients VAS and Laitinen modified scale were taken before and after treatment. RESULTS: After 2-weeks therapy there was improvement in the VAS and Laitinen scale (all components) in both groups, except for the part of the Laitinen scale on the reduction of activity in the group II (TENS). There was no statistically significant difference between the TENS and IF groups in reducing the intensity and other aspects of pain (frequency, pain medication and activity limitation) under the influence of therapy (p > 0.05). CONCLUSIONS: Interferential current and TENS therapy are effective for pain relief in patients with low back pain. The study showed equal analgesic efficacy of both treatments.

[The effect of selected physical procedures on mobility in women with rheumatoid arthritis]. / Wplyw wybranych zabieg6w fizykalnych na sprawnoscc ruchowa kobiet z reumatoidalnym zapaleniem stawów.

UNLABELLED: Electrotherapy, including iontophoresis and magnetic field, is one of the most commonly used physical procedures in the treatment of rheumatoid arthritis (RS). THE AIM OF THE PAPER: To evaluate the effect of iontophoresis and magnetic field procedures on the intensity and frequency of pain sensation, administration of analgesics, limitation of knee joint mobility and comparative evaluation of analgesic effect s of the applied procedures. MATERIAL AND METHODS: The study included a group of 60 female patients affected by RS with knee joint pain. Patients were randomly assigned to 3 equally-numbered groups. Group I was subjected to 20 iontophoresis procedures. Group II underwent 20 procedures with magnetic field. Group III was treated with 20 procedures combining both iontophoresis and magnetic field. Each iontophoresis procedure lasted 20 minutes, whereas the magnetic field procedure took 30 minutes. All study participants were evaluated in relation to pain sensation after and before the treatment with VAS (Visual Analogue Scale) and Latinen scale. RESULTS: After a 4-week therapy in all the three groups there was a statistically significant decrease in pain perception with VAS scale and with all domains of Laitinen scale excluding the limitation of physical activity criterion. The comparative evaluation of statistically important differences after the therapy between the groups revealed marked decrease of pain perception in groups I and II comparing to group II. There were no significant differences between groups I and III. CONCLUSIONS: Iontophoresis and magnetic field treatments demonstrate effective analgesic property in female patients with rheumatoid arthritis. The conducted studies showed the highest analgesic effects for both treatments used.